Affordable Access

Carbon source-dependent transcriptional regulation of the mitochondrial glycerol-3-phosphate dehydrogenase gene, GUT2, from Saccharomyces cerevisiae.

Authors
  • Grauslund, M
  • Rønnow, B
Type
Published Article
Journal
Canadian journal of microbiology
Publication Date
Dec 01, 2000
Volume
46
Issue
12
Pages
1096–1100
Identifiers
PMID: 11142398
Source
Medline
License
Unknown

Abstract

Cytosolic glycerol kinase (Gut1p) and mitochondrial glycerol-3-phosphate dehydrogenase (Gut2p) constitute the glycerol utilization pathway in Saccharomyces cerevisiae. Transcriptional analysis of the GUT2 gene showed that it was repressed by glucose and derepressed on the non-fermentable carbon sources, glycerol, lactate and ethanol. Derepression of GUT2 requires the protein kinase Snflp as well as the heteromeric protein complex, Hap2/3/4/5, and its putative DNA-binding site (UASHAP) located in the promoter region. Furthermore, glucose repression of GUT2 requires the negative regulator, Opi1p.

Report this publication

Statistics

Seen <100 times