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Carbon nanotag based visual detection of influenza A virus by a lateral flow immunoassay

Authors
  • Wiriyachaiporn, Natpapas1
  • Sirikett, Hathainan1
  • Maneeprakorn, Weerakanya1
  • Dharakul, Tararaj1, 2
  • 1 National Nanotechnology Center (NANOTEC), National Science and Technology Development Agency (NSTDA), PathumThani, 12120, Thailand , PathumThani (Thailand)
  • 2 Faculty of Medicine Siriraj Hospital, Mahidol University, Bangkok, 10700, Thailand , Bangkok (Thailand)
Type
Published Article
Journal
Microchimica Acta
Publisher
Springer-Verlag
Publication Date
Mar 27, 2017
Volume
184
Issue
6
Pages
1827–1835
Identifiers
DOI: 10.1007/s00604-017-2191-6
Source
Springer Nature
Keywords
License
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Abstract

The authors report on a rapid and direct visual test for the detection of influenza A virus using a carbon nanotag based lateral flow assay. Carbon nanoparticles in the form of nanostrings are acting as reporters. As carbon nanotags accumulate in the test zone due to formation of an antibody-antigen-carbon nanotag antibody complex, and this allows for the direct visualization of the analytical signal. Under optimal conditions, influenza A virus can be determined in allantoic fluid inoculated with the virus with a limit of detection of 350 TCID50.mL−1 (i.e., the 50% tissue culture infectious dose). No interference is found for several other tested proteins, and for closely related viruses. Cell lysates containing different seasonal strains of influenza A viruses (including the H1N1 and H3N2 strains) collected from clinical samples were analyzed. It is demonstrated that the method can detect both influenza A viruses without interference by biological matrices. In our perception, this method has a wide potential in that it may be extended to a generally applicable platform for rapid diagnosis influenza A viruses. Graphical abstractSchematic of a rapid and direct visual test for the detection of influenza A virus using a carbon nanotag based lateral flow immunoassay. The presence of the virus induces an accumulation of the carbon nanotag conjugates, thereby providing a signal visualization.

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