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Carbon black nanoparticles induce type II epithelial cells to release chemotaxins for alveolar macrophages

Authors
  • Barlow, Peter G1, 2
  • Clouter-Baker, Anna2
  • Donaldson, Ken3
  • MacCallum, Janis2
  • Stone, Vicki2
  • 1 M.R.C/University of Edinburgh Centre for Inflammation Research, Queen's Medical Research Institute, 47 Little France Crescent, Edinburgh, EH16 4TJ, UK , Edinburgh
  • 2 Napier University, Biomedicine Research Group, 10 Colinton Road, Edinburgh, EH10 5DT, UK , Edinburgh
  • 3 M.R.C/University of Edinburgh Centre for Inflammation Research, Queen's Medical Research Institute, ELEGI/Colt Laboratories, 47 Little France Crescent, Edinburgh, EH16 4TJ, UK , Edinburgh
Type
Published Article
Journal
Particle and Fibre Toxicology
Publisher
BioMed Central
Publication Date
Dec 06, 2005
Volume
2
Issue
1
Identifiers
DOI: 10.1186/1743-8977-2-11
Source
Springer Nature
Keywords
License
Yellow

Abstract

BackgroundAlveolar macrophages are a key cell in dealing with particles deposited in the lungs and in determining the subsequent response to that particle exposure. Nanoparticles are considered a potential threat to the lungs and the mechanism of pulmonary response to nanoparticles is currently under intense scrutiny. The type II alveolar epithelial cell has previously been shown to release chemoattractants which can recruit alveolar macrophages to sites of particle deposition. The aim of this study was to assess the responses of a type II epithelial cell line (L-2) to both fine and nanoparticle exposure in terms of secretion of chemotactic substances capable of inducing macrophage migration.ResultsExposure of type II cells to carbon black nanoparticles resulted in significant release of macrophage chemoattractant compared to the negative control and to other dusts tested (fine carbon black and TiO2 and nanoparticle TiO2) as measured by macrophage migration towards type II cell conditioned medium. SDS-PAGE analysis of the conditioned medium from particle treated type II cells revealed that a higher number of protein bands were present in the conditioned medium obtained from type II cells treated with nanoparticle carbon black compared to other dusts tested. Size-fractionation of the chemotaxin-rich supernatant determined that the chemoattractants released from the epithelial cells were between 5 and 30 kDa in size.ConclusionThe highly toxic nature and reactive surface chemistry of the carbon black nanoparticles has very likely induced the type II cell line to release pro-inflammatory mediators that can potentially induce migration of macrophages. This could aid in the rapid recruitment of inflammatory cells to sites of particle deposition and the subsequent removal of the particles by phagocytic cells such as macrophages and neutrophils. Future studies in this area could focus on the exact identity of the substance(s) released by the type II cells in response to particle exposure.

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