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Carbohydrate composition of specific rat epididymal protein.

Authors
  • Garberi, J C
  • Fontana, J D
  • Blaquier, J A
Type
Published Article
Journal
International journal of andrology
Publication Date
Dec 01, 1982
Volume
5
Issue
6
Pages
619–626
Identifiers
PMID: 7160923
Source
Medline
License
Unknown

Abstract

Previous results demonstrated that androgen-dependent rat specific epididymal proteins (SEP) were bound to spermatozoa during their maturation in the epididymis. This paper describes the purification of glycoprotein DE, which constitutes 40% of SEP, and the identification and semiquantitative determination of the sugars forming its oligosaccharide chain. Affinity chromatography on Sepharose-Concanavalin A produced a sample of D-E 95% pure in which 10.5 g of sugar were present per 100 g protein. The percentual composition of the oligosaccharide was D-mannose 19%; D-galactose 3%; N-acetyl-D-glucosamine 33%; N-acetyl-neuraminic acid 31% and D-glucose 13%.

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