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Caracterização do complexo coesina de Trypanosoma cruzi

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Repositório Institucional da Universidade de Brasília
Keywords
  • Microbiologia Molecular
  • Tripanossoma Cruzi - Citologia
  • Leishmaniose
  • Tripanossoma Cruzi
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Abstract

The segregation of sister chromatids to opposite poles of the cell during division is the most complex and, at the same time, the most important event during the life cycle of a eukaryotic cell. Both in mitosis and meiosis cohesion between sister chromatids is essential for the occurrence of the correct chromosomal segregation. The protein complex responsible for cohesion between chromatids is called Cohesin. The Cohesin complex is well known in yeast and mammals, consisting of two SMC (structural maintenance of chromosomes) proteins, SMC1 and SMC3, and two proteins SCC (sister chromatid cohesion) proteins, the SCC1 and SCC3 (SA1 and SA2 in mammalian cells). The Cohesin keeps sister chromatids together from S phase until the transition between metaphase and anaphase in cell cycle, when sister chromatids separate to the opposite poles of the cell. In trypanosomatids, there are few studies about this complex and the genome project revealed the presence of all Cohesin complex genes in Trypanosoma cruzi, Trypanosoma brucei and Leishmania major. In this work we proposed the analysis of the expression of the Cohesin subunits and its imunocytolocalization in T. cruzi cells. An antibody anti-TcSCC1 produced in rabbit, was used in western blots and immunofluorescence confocal microscopy analyses of amastigote, epimastigote and trypomastigote forms of T. cruzi. These analyses indicate that the TcSCC1 protein is detected mainly in the amastigote forms with distinct pattern of nucleus localization. Epimastigote form presented a weak signal for the anti-SCC1 antibody and trypomastigote form presented no signal. These results suggest that the SCC1 subunit of the Cohesin complex is present in T. cruzi and it is mainly evident in the nucleus of amastigote form of this parasite. The expression levels of each subunit of the Cohesin complex were analyzed by real time RT-PCR assays in the three forms of T. cruzi and it was found few differences between these levels, suggesting a post-transcriptional control in the SCC1 expression in T. cruzi.

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