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Capillary column gas-liquid chromatographic resolution of oxidized cholesterol derivatives

Authors
  • Park, S.Won
  • Addis, P.B.
Type
Published Article
Journal
Analytical Biochemistry
Publisher
Elsevier BV
Publication Date
Jan 01, 1985
Volume
149
Issue
1
Pages
275–283
Identifiers
DOI: 10.1016/0003-2697(85)90505-6
Source
Elsevier
Keywords
License
Unknown

Abstract

Fused-silica capillary columns were evaluated for the resolution of oxidized cholesterol derivatives. Thermal instability of diol derivatives, epimeric 7α- and 7β-hydroxy, 4β-hydroxy, and 25-hydroxycholesterol, was observed during gas chromatography. After derivatization as trimethylsilyl ethers the foregoing diols, α-epoxide, cholestane-triol, 7-ketocholesterol, and cholesta-3,5-dien-7-one were completely resolved on a DB-1 column. Each oxidized sterol revealed excellent response linearity as the trimethylsilylated sterol, enabling reliable quantification. The identity of each derivatized sterol was confirmed by mass spectrometry.

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