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Canine C-reactive protein measurements in cerebrospinal fluid by a time-resolved immunofluorimetric assay.

Authors
  • Martínez-Subiela, Silvia1
  • Caldin, Marco
  • Parra, Maria Dolores
  • Ottolini, Nicola
  • Bertolini, Giovanna
  • Bernal, Luis J
  • García-Martinez, Juan D
  • Cerón, Jose J
  • 1 Animal Medicine and Surgery Department, Faculty of Veterinary Medicine, University of Murcia, 30100 Espinardo Campus, Murcia, Spain. , (Spain)
Type
Published Article
Journal
Journal of veterinary diagnostic investigation : official publication of the American Association of Veterinary Laboratory Diagnosticians, Inc
Publication Date
Jan 01, 2011
Volume
23
Issue
1
Pages
63–67
Identifiers
PMID: 21217029
Source
Medline
Language
English
License
Unknown

Abstract

In the current study, the quantification of C-reactive protein (CRP) in cerebrospinal fluid (CSF) of dogs using an adapted time-resolved immunofluorimetric assay (TR-IFMA) was investigated, as well as whether the assay could be used to detect the range of CRP concentrations found in different clinical situations. Intra- and interassay coefficients of variation were below 15% in all cases. The TR-IFMA measured the CRP values in a proportional and linear manner (r = 0.99); also CRP concentrations measured in CSF and in serum were significantly correlated (r = 0.80, P = 0.003). The limit of detection of the method was 7.1 × 10(-6) mg/l. The assay was able to detect differences in CRP concentrations in CSF of dogs with inflammatory disorders compared with dogs with spinal cord compression or idiopathic epilepsy. In conclusion, TR-IFMA constitutes a very sensitive, precise, and accurate method for the measurement of CRP concentrations in CSF.

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