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Candidate gene mapping identifies genomic variations in the fire blight susceptibility genes HIPM and DIPM across the Malus germplasm

Authors
  • Tegtmeier, Richard1
  • Pompili, Valerio2
  • Singh, Jugpreet1
  • Micheletti, Diego2
  • Silva, Katchen Julliany Pereira1
  • Malnoy, Mickael2
  • Khan, Awais1
  • 1 Cornell University, Geneva, NY, 14456, USA , Geneva (United States)
  • 2 Fondazione Edmund Mach, San Michele all’Adige, Italy , San Michele all’Adige (Italy)
Type
Published Article
Journal
Scientific Reports
Publisher
Springer Nature
Publication Date
Oct 01, 2020
Volume
10
Issue
1
Identifiers
DOI: 10.1038/s41598-020-73284-w
Source
Springer Nature
License
Green

Abstract

Development of apple (Malus domestica) cultivars resistant to fire blight, a devastating bacterial disease caused by Erwinia amylovora, is a priority for apple breeding programs. Towards this goal, the inactivation of members of the HIPM and DIPM gene families with a role in fire blight susceptibility (S genes) can help achieve sustainable tolerance. We have investigated the genomic diversity of HIPM and DIPM genes in Malus germplasm collections and used a candidate gene-based association mapping approach to identify SNPs (single nucleotide polymorphisms) with significant associations to fire blight susceptibility. A total of 87 unique SNP variants were identified in HIPM and DIPM genes across 93 Malus accessions. Thirty SNPs showed significant associations (p < 0.05) with fire blight susceptibility traits, while two of these SNPs showed highly significant (p < 0.001) associations across two different years. This research has provided knowledge about genetic diversity in fire blight S genes in diverse apple accessions and identified candidate HIPM and DIPM alleles that could be used to develop apple cultivars with decreased fire blight susceptibility via marker-assisted breeding or biotechnological approaches.

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