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Calyculin A retraction of mature megakaryocytes proplatelets from embryonic stem cells

Authors
  • Tamaru, Satoshi
  • Kitajima, Kenji
  • Nakano, Tohru
  • Eto, Koji
  • Yazaki, Akira
  • Kobayashi, Toshihiko
  • Matsumoto, Takeshi
  • Wada, Hideo
  • Katayama, Naoyuki
  • Nishikawa, Masakatsu
Type
Published Article
Journal
Biochemical and Biophysical Research Communications
Publication Date
Jan 01, 2007
Volume
366
Issue
3
Pages
763–768
Identifiers
DOI: 10.1016/j.bbrc.2007.12.032
Source
Elsevier
Keywords
License
Unknown

Abstract

Platelets are produced by megakaryocytes (MKs) through proplatelet formation (PPF), or cytoplasmic extensions, in vitro. Through the use of video-enhanced light microscopy, as well as localization of cytoskeletal proteins by confocal microscopy, the reaction of fully mature MK proplatelets, derived from murine embryonic stem cells, to various agents was studied. Calyculin A (protein phosphatase 1/2A inhibitor) treatment induced proplatelet retraction. In MKs with PPF, the expression of actin, myosin IIA, monophosphorylated myosin light chain (MLC-P1), and diphosphorylated myosin light chain (MLC-P2) was diffusely located. Following calyculin A treatment, actin was diffusely localized in retracted MKs and was expressed particularly in the periphery. MLC-P1 was also localized primarily in the periphery; however, MLC-P2 was expressed mostly in the inner area of proplatelets. Protein phosphatase inhibitors may result in increased hyperphosphorylation of localized MLC, which could alter the balance of actomyosin force in a cell, and therefore induce proplatelets retraction.

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