Affordable Access

Access to the full text

The Calvin cycle enzyme sedoheptulose-1,7-bisphosphatase is encoded by a light-regulated gene in Chlamydomonas reinhardtii

Authors
  • Hahn, Daniela1
  • Kaltenbach, Carmen1
  • Kück, Ulrich1
  • 1 Ruhr Universität Bochum, Lehrstuhl für Allgemeine Botanik, Bochum, 44780, Germany , Bochum
Type
Published Article
Journal
Plant Molecular Biology
Publisher
Kluwer Academic Publishers
Publication Date
Apr 01, 1998
Volume
36
Issue
6
Pages
929–934
Identifiers
DOI: 10.1023/A:1005911022601
Source
Springer Nature
Keywords
License
Yellow

Abstract

We have studied the light-dependent expression of the Chlamydomonas reinhardtii csbp gene encoding sedoheptulose-1,7-bisphosphatase (SBPase), an enzyme of the pentose-phosphate pathway. Expression studies using light/dark-synchronized cultures revealed that csbp mRNA abundance increases significantly during illumination. We have used a 1.4 kb region upstream of the csbp gene in transcriptional fusions to the homologous arylsulfatase-encoding reporter gene (ars). In transformants carrying the chimeric csbp/ars reporter gene, arylsulfatase activity is detected in the absence of sulfate, a condition under which the endogenous ars gene is repressed. Moreover, ars mRNA accumulation is dramatically stimulated by light, indicating that 1.4 kb of the csbp 5′-untranslated region are sufficient to confer light-dependent expression on the ars reporter gene.

Report this publication

Statistics

Seen <100 times