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Calmodulin activates nuclear protein import: a link between signal transduction and nuclear transport.

Authors
  • Sweitzer, T D
  • Hanover, J A
Type
Published Article
Journal
Proceedings of the National Academy of Sciences of the United States of America
Publication Date
Dec 10, 1996
Volume
93
Issue
25
Pages
14574–14579
Identifiers
PMID: 8962094
Source
Medline
License
Unknown

Abstract

In addition to the well-characterized GTP-dependent nuclear transport observed in permeabilized cells, we detected a mode of nuclear transport that was GTP-independent at elevated cytoplasmic calcium concentrations. Nuclear transport under these conditions was blocked by calmodulin inhibitors. Recombinant calmodulin restored ATP-dependent nuclear transport in the absence of cytosol. Calmodulin-dependent transport was inhibited by wheat germ agglutinin consistent with transport proceeding through nuclear pores. We propose that release of intracellular calcium stores upon cell activation inhibits GTP-dependent nuclear transport; the elevated cytosolic calcium then acts through calmodulin to stimulate the novel GTP-independent mode of import.

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