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Calcium-permeable cation channels are involved in uranium uptake in Arabidopsis thaliana.

Authors
  • Sarthou, Manon C M1
  • Devime, Fabienne1
  • Baggio, Célia1
  • Figuet, Sylvie1
  • Alban, Claude1
  • Bourguignon, Jacques2
  • Ravanel, Stéphane3
  • 1 Univ. Grenoble Alpes, INRAE, CEA, CNRS, IRIG, LPCV, 38000 Grenoble, France. , (France)
  • 2 Univ. Grenoble Alpes, INRAE, CEA, CNRS, IRIG, LPCV, 38000 Grenoble, France. Electronic address: [email protected] , (France)
  • 3 Univ. Grenoble Alpes, INRAE, CEA, CNRS, IRIG, LPCV, 38000 Grenoble, France. Electronic address: [email protected] , (France)
Type
Published Article
Journal
Journal of hazardous materials
Publication Date
Oct 06, 2021
Volume
424
Issue
Pt B
Pages
127436–127436
Identifiers
DOI: 10.1016/j.jhazmat.2021.127436
PMID: 34638071
Source
Medline
Keywords
Language
English
License
Unknown

Abstract

Uranium (U) is a non-essential and toxic element that is taken up by plants from the environment. The assimilation pathway of U is still unknown in plants. In this study, we provide several evidences that U is taken up by the roots of Arabidopsis thaliana through Ca2+-permeable cation channels. First, we showed that deprivation of Arabidopsis plants with calcium induces a 1.5-fold increase in the capacity of roots to accumulate U, suggesting that calcium deficiency promotes the radionuclide import pathway. Second, we showed that external calcium inhibits U accumulation in roots, suggesting a common route for the uptake of both cations. Third, we found that gadolinium, nifedipine and verapamil inhibit the absorption of U, suggesting that different types of Ca2+-permeable channels serve as a route for U uptake. Last, we showed that U bioaccumulation in Arabidopsis mutants deficient for the Ca2+-permeable channels MCA1 and ANN1 is decreased by 40%. This suggests that MCA1 and ANN1 contribute to the absorption of U in different zones and cell layers of the root. Together, our results describe for the first time the involvement of Ca2+-permeable cation channels in the cellular uptake of U. Copyright © 2021 Elsevier B.V. All rights reserved.

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