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Calcium signaling mediates five types of cell morphological changes to form neural rosettes.

Authors
  • Hříbková, Hana1
  • Grabiec, Marta1
  • Klemová, Dobromila2
  • Slaninová, Iva1
  • Sun, Yuh-Man3
  • 1 Department of Biology, Faculty of Medicine, Masaryk University, Brno 62500, Czech Republic. , (Czechia)
  • 2 Department of Histology and Embryology, Faculty of Medicine, Masaryk University, Brno 62500, Czech Republic. , (Czechia)
  • 3 Department of Biology, Faculty of Medicine, Masaryk University, Brno 62500, Czech Republic [email protected] , (Czechia)
Type
Published Article
Journal
Journal of Cell Science
Publisher
The Company of Biologists
Publication Date
Feb 12, 2018
Volume
131
Issue
3
Identifiers
DOI: 10.1242/jcs.206896
PMID: 29361526
Source
Medline
Keywords
License
Unknown

Abstract

Neural rosette formation is a critical morphogenetic process during neural development, whereby neural stem cells are enclosed in rosette niches to equipoise proliferation and differentiation. How neural rosettes form and provide a regulatory micro-environment remains to be elucidated. We employed the human embryonic stem cell-based neural rosette system to investigate the structural development and function of neural rosettes. Our study shows that neural rosette formation consists of five types of morphological change: intercalation, constriction, polarization, elongation and lumen formation. Ca2+ signaling plays a pivotal role in the five steps by regulating the actions of the cytoskeletal complexes, actin, myosin II and tubulin during intercalation, constriction and elongation. These, in turn, control the polarizing elements, ZO-1, PARD3 and β-catenin during polarization and lumen production for neural rosette formation. We further demonstrate that the dismantlement of neural rosettes, mediated by the destruction of cytoskeletal elements, promotes neurogenesis and astrogenesis prematurely, indicating that an intact rosette structure is essential for orderly neural development.

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