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Calcium phosphate nanoneedle based gene delivery system for cancer genetic immunotherapy.

Authors
  • Zhao, Jing1
  • Chen, Guochuang2
  • Pang, Xiaojuan2
  • Zhang, Peifa3
  • Hou, Xiaohu2
  • Chen, Ping2
  • Xie, Yi-Wu3
  • He, Cheng-Yi2
  • Wang, Zhiyong4
  • Chen, Zhi-Ying5
  • 1 Scientific Research Center, The Seventh Affiliated Hospital, Sun Yat-sen University, Shenzhen, 18107, PR China; Laboratory for Gene and Cell Engineering, Shenzhen Institutes of Advanced Technology, Chinese Academy of Sciences, Shenzhen, 518055, PR China. , (China)
  • 2 Laboratory for Gene and Cell Engineering, Shenzhen Institutes of Advanced Technology, Chinese Academy of Sciences, Shenzhen, 518055, PR China; Syno Minicircle Biotechnology Co. Ltd., Shenzhen, 518055, PR China. , (China)
  • 3 Syno Minicircle Biotechnology Co. Ltd., Shenzhen, 518055, PR China. , (China)
  • 4 Scientific Research Center, The Seventh Affiliated Hospital, Sun Yat-sen University, Shenzhen, 18107, PR China; School of Materials Science and Engineering, Center for Functional Biomaterials, Key Laboratory for Polymeric Composite and Functional Materials of Ministry of Education, Sun Yat-sen University, Guangzhou, 510275, China. Electronic address: [email protected] , (China)
  • 5 Laboratory for Gene and Cell Engineering, Shenzhen Institutes of Advanced Technology, Chinese Academy of Sciences, Shenzhen, 518055, PR China; Syno Minicircle Biotechnology Co. Ltd., Shenzhen, 518055, PR China. Electronic address: [email protected] , (China)
Type
Published Article
Journal
Biomaterials
Publication Date
Aug 01, 2020
Volume
250
Pages
120072–120072
Identifiers
DOI: 10.1016/j.biomaterials.2020.120072
PMID: 32361307
Source
Medline
Keywords
Language
English
License
Unknown

Abstract

Ovarian cancer has become one of the most common gynecological cancers with a high mortality. However, conventional surgery together with combination chemotherapy is difficult to achieve ideal therapeutic effect. Although genetic immunotherapy is applied to active immune responses against cancer, the absence of efficient in vivo gene delivery technique is still an obstacle in clinical application. To overcome these problems, a minicircle DNA vector encoding humanized anti-EpCAM/CD3 bispecific antibody (BsAbEPH) has been constructed. Moreover, different shapes of calcium phosphate (CaPO) biomaterials were prepared. Specifically, the CaPO-nanoneedle-mediated "cell perforation" transfection technology achieves high levels of gene expression in peritoneal cavity. In an intraperitoneal xenograft model with human ovarian cancer cell line SKOV3, the CaPO-nanoneedle/minicircle DNA system expressed BsAbEPH resulted in significant retardation of cancer growth and extension of mouse life-span with limited toxicity. And this system can be made as off-the-shelf and easy-to-use products. Therefore, CaPO-nanoneedle based non-viral gene delivery technology will have great potential in clinical application. Copyright © 2020 Elsevier Ltd. All rights reserved.

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