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Calcium-loaded microsomes from uterine smooth muscle. A biological system to test for calcium ionophore action of prostaglandins.

Authors
  • Carsten, M E
  • Miller, J D
Type
Published Article
Journal
Gynecologic and obstetric investigation
Publication Date
Jan 01, 1984
Volume
17
Issue
2
Pages
78–83
Identifiers
PMID: 6423463
Source
Medline
License
Unknown

Abstract

Smooth muscle microsomal vesicles were loaded with calcium in the presence of oxalate and ATP. The intact vesicles that contained calcium oxalate crystals were separated by ultracentrifugation from empty vesicles. This results in a unique model system, composed of a biologically active membrane with virtually all of the calcium inside. Intravesicular calcium is differentiated from externally bound calcium using ionophore X537A and EGTA. EGTA released calcium slowly with a half time of 93 min. The ionophore X537A rapidly released calcium with a half time of 12 min. This model can be used to test for calcium ionophoretic action. Prostaglandin (PG) E2 and prostacyclin (PGI2) were tested in this system. We found that PGE2 and PGI2 did not change calcium permeability.

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