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Calcium imaging reveals nicotinic acetylcholine receptors on cultured mushroom body neurons.

Authors
  • Bicker, G
  • Kreissl, S
Type
Published Article
Journal
Journal of neurophysiology
Publication Date
Feb 01, 1994
Volume
71
Issue
2
Pages
808–810
Identifiers
PMID: 8176442
Source
Medline
License
Unknown

Abstract

1. We used fluorescence imaging with the visible wavelength indicator fluo-3 to investigate the calcium responses to cholinergic ligands of honeybee Kenyon cells in primary culture. 2. Application of acetylcholine (ACh) or nicotine, but not pilocarpine, promoted a calcium influx into the cell body and neurites. The increase in intracellular calcium after ACh stimulation was blocked by alpha-bungarotoxin. These results support previous histochemical studies that suggested the expression of nicotinic cholinergic receptors on Kenyon cells. 3. After depolarization with high K+ solution fluorescence increased in the somata and neurites, which indicates the presence of voltage-gated Ca2+ channels in Kenyon cell membranes.

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