Affordable Access

Calcium-dependent release of accumulated glutamate from synaptic vesicles within permeabilized nerve terminals.

Authors
  • Kish, P E
  • Ueda, T
Type
Published Article
Journal
Neuroscience Letters
Publisher
Elsevier
Publication Date
Jan 28, 1991
Volume
122
Issue
2
Pages
179–182
Identifiers
PMID: 1902921
Source
Medline
License
Unknown

Abstract

We have studied glutamate release from synaptic vesicles in permeabilized synaptosomes, which were preloaded with [3H]glutamate in an ATP-dependent manner. The release was found to be calcium-dependent and to require a heat-labile cytosolic macromolecule factor for maximum activity. Maximal release occurred at 5 microM free Ca2+ and within 5 min. Of the other divalent cations tested, only barium stimulated release of vesicular glutamate. The release was inhibited by N-ethylmaleimide. These results are characteristic of exocytotic release of monoamines and peptides observed in endocrine systems, and constitute direct evidence for the notion that calcium-dependent release of glutamate originates from the vesicular pool.

Report this publication

Statistics

Seen <100 times