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Calcium and Vitamin D increase mRNA levels for the growth control hIK1 channel in human epidermal keratinocytes but functional channels are not observed

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BioMed Central
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PMC
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  • Research Article
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  • Biology
  • Chemistry

Abstract

1471-5945-4-7.fm ral ss BioMed CentBMC Dermatology Open AcceResearch article Calcium and Vitamin D increase mRNA levels for the growth control hIK1 channel in human epidermal keratinocytes but functional channels are not observed Vlasios Manaves1, Wuxuan Qin1, Amy L Bauer1, Sandra Rossie2, Masakazu Kobayashi3 and Stanley G Rane*1,4 Address: 1Molecular Human Physiology Group, Fujisawa Research Institute of America, Evanston, IL 60201 USA, 2Department of Biochemistry, Purdue University, West Lafayette, IN 47907 USA, 3Fujisawa Research Institute of America, Evanston, IL 60201 USA and 4Depts. of Medicine, Evanston Northwestern Healthcare, Evanston, IL 60201, and Northwestern University Medical School, Chicago, IL 60611 USA Email: Vlasios Manaves - [email protected]; Wuxuan Qin - [email protected]; Amy L Bauer - [email protected]; Sandra Rossie - [email protected]; Masakazu Kobayashi - [email protected]; Stanley G Rane* - [email protected] * Corresponding author Abstract Background: Intermediate-conductance, calcium-activated potassium channels (IKs) modulate proliferation and differentiation in mesodermal cells by enhancing calcium influx, and they contribute to the physiology of fluid movement in certain epithelia. Previous reports suggest that IK channels stimulate proliferative growth in a keratinocyte cell line; however, because these channels indirectly promote calcium influx, a critically unique component of the keratinocyte differentiation program, an alternative hypothesis is that they would be anti- proliferative and pro-differentiating. This study addresses these hypotheses. Methods: Real-time PCR, patch clamp electrophysiology, and proliferation assays were used to determine if human IK1 (hIK1) expression and function are correlated with either proliferation or differentiation in cultured human skin epidermal keratinocytes, and skin biopsies grown in explant culture. Results: hIK1 mRNA expression in human keratinocytes a

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