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Caffeine dissociates complexes between DNA and intercalating dyes: application for bleaching fluorochrome-stained cells for their subsequent restaining and analysis by laser scanning cytometry.

Authors
  • Bedner, E
  • Du, L
  • Traganos, F
  • Darzynkiewicz, Z
Type
Published Article
Journal
Cytometry
Publication Date
Jan 01, 2001
Volume
43
Issue
1
Pages
38–45
Identifiers
PMID: 11122483
Source
Medline
License
Unknown

Abstract

Solutions of CFN can dissociate the DNA-bound PI, 7-AAD, EB, and possibly other intercalating fluorochromes. The bleached cells can be restained and reanalyzed by LSC. This approach can also be used to remove such fluorochromes from nucleic acids immobilized in gels and perhaps in other solid matrices. Analysis of the kinetics of fluorochrome removal from cells can possibly be used to study their binding affinities to nucleic acids in situ.

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