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Induction of Protein Oxidation in Human Low Lipoprotein by the Photosensitive Organic Hydroperoxide,N,N′-bis(2-Hydroxyperoxy-2-Methoxyethyl)-1,4,5,8-Naphthalene-Tetra-Carboxylic-Diimide

Authors
Journal
Biochemical and Biophysical Research Communications
0006-291X
Publisher
Elsevier
Publication Date
Volume
206
Issue
1
Identifiers
DOI: 10.1006/bbrc.1995.1020
Disciplines
  • Biology
  • Physics

Abstract

Abstract We have developed a new molecular probe, N,N′-bis(2-hydroxyperoxy-2-methoxyethyl)-1,4,5,8 -naphthalene-tetra-carboxylic-diimide (NP-III), that specifically generates hydroxyl radical upon irradiation with longer wavelength ultraviolet light (UVA). Hydroxyl radicals are generated only upon irradiation, thus NP-III is a new controllable hydroxyl radical source. Apolipoprotein (apo-B) of human low density lipoprotein (LDL), and bovine serum alubumin (BSA), were irradiated with UVA in the presence of NP-III and their oxidation was evaluated by two independent methods: assay of protein carbonyl groups and gel electrophoresis. NP-III oxidized apo-B and BSA in a time- and concentration-dependent manner. The results demonstrate that NP-III is a contollable, precise, and potentially tagetable source of hydroxyl radicals with which to induce protein oxidation.

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