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Genetically-engineered bacteria expressing human enzymes and their use in the study of mutagens and mutagenesis

Authors
Journal
Toxicology
0300-483X
Publisher
Elsevier
Publication Date
Identifiers
DOI: 10.1016/s0300-483x(02)00292-5
Keywords
  • Escherichia Coli
  • Salmonella Typhimurium
  • Mutagenicity
  • Bioactivation
  • P450
  • N-Acetyltransferase
Disciplines
  • Biology

Abstract

Abstract Expression of human enzymes in bacterial cells has made possible the development of a new generation of mutagenicity assays. Metabolic activation takes place within the internal milieu of the target cell, so that no membrane barrier is interposed between the reactive metabolite and the DNA target. This technology has been extended to all major classes of enzymes of xenobiotic biotransformation, including P450. Expression of sequence variants of mutagen-bioactivation enzymes provides a new tool for studying their structure and function.

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