Abstract Non-equilibrium thermodynamics of electrokinetic effects, like hydraulic permeability, electroosmosis, streaming potential and current of water across membranes containing lipid pairs, has been studied. The lipid pairs chosen were cephalin + phosphatidyl serine (serine), cephalin + lecithin, cephalin + phosphatidyl inositol (inositol), serine + inositol, lecithin + serine and lecithin + inositol. L coefficients for water/mixed-lipid systems have been calculated and near equality of the cross coefficients L 12 and L 21 proved the validity of the Onsager reciprocity relation. All the electrokinetic effects have been found to differ in magnitude from those obtained for single-lipid membranes. Efficiency of electrokinetic energy conversion has also been calculated.