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A dot screening procedure on nitrocellulose for detecting anti-idiotopes in hybridoma supernatants

Authors
Journal
Journal of Immunological Methods
0022-1759
Publisher
Elsevier
Publication Date
Volume
89
Issue
2
Identifiers
DOI: 10.1016/0022-1759(86)90362-5
Keywords
  • Nitrocellulose
  • Alkaline Phosphatase
  • Anti-Idiotope

Abstract

Abstract A dot assay on nitrocellulose was developed for screening hybridoma fusions for the presence of anti-idiotopes. The technique involves spotting of hybridoma supernatants onto nitrocellulose filters, and detection of anti-idiotope with an alkaline phosphatase-coupled idiotype.

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