Affordable Access

Publisher Website

A dot screening procedure on nitrocellulose for detecting anti-idiotopes in hybridoma supernatants

Authors
Journal
Journal of Immunological Methods
0022-1759
Publisher
Elsevier
Publication Date
Volume
89
Issue
2
Identifiers
DOI: 10.1016/0022-1759(86)90362-5
Keywords
  • Nitrocellulose
  • Alkaline Phosphatase
  • Anti-Idiotope

Abstract

Abstract A dot assay on nitrocellulose was developed for screening hybridoma fusions for the presence of anti-idiotopes. The technique involves spotting of hybridoma supernatants onto nitrocellulose filters, and detection of anti-idiotope with an alkaline phosphatase-coupled idiotype.

There are no comments yet on this publication. Be the first to share your thoughts.

Statistics

Seen <100 times
0 Comments

More articles like this

A dot screening procedure on nitrocellulose for de...

on Journal of Immunological Metho... May 22, 1986

An improved dot immunobinding assay for screening...

on Journal of Immunological Metho... Jan 24, 1991

An improved dot immunobinding assay for screening...

on Journal of Immunological Metho... Jan 01, 1991

An economical procedure for screening of hybridoma...

on Tropical medicine and parasito... June 1990
More articles like this..