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Histamine H1-receptor-like binding sites in the locust nervous tissue

Authors
Journal
Neuroscience Letters
0304-3940
Publisher
Elsevier
Publication Date
Volume
116
Issue
3
Identifiers
DOI: 10.1016/0304-3940(90)90096-r
Keywords
  • Locust
  • Locusta Migratoria
  • Insect
  • Histamine
  • H1-Receptor
  • [3H]Mianserin Binding Site

Abstract

Abstract The histamine H 1-receptor-like binding sites in the nervous tissue of the locust Locusta migratoria were investigated with a conventional radio-receptor assay using [ 3H]mianserin as the radio ligand. Binding of [ 3H]mianserin to the binding site is sensitive to proteases and heat treatment. It shows the characteristics of ligand-receptor interactions. The single binding site has high affinity for mianserin (K D = 7.05 nM) and is present in high concentrations (B max = 1.53 pmol/mg) in the whole nervous tissue. All tested antihistamines have a high affinity for the binding site, which suggests that it represents an insect histamine receptor. Nevertheless, it shows its peculiarities distinguishing it from vertebrate histamine H 1-receptors.

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