Affordable Access

Publisher Website

Activation of phosphodiesterase in frog rod outer segment by an intermediate of rhodopsin photolysis. I.

Authors
Journal
Biochimica et Biophysica Acta (BBA) - General Subjects
0304-4165
Publisher
Elsevier
Publication Date
Volume
675
Issue
2
Identifiers
DOI: 10.1016/0304-4165(81)90225-7
Keywords
  • Phosphodiesterase Activation
  • Rhodopsin Photolysis
  • Cyclic Gmp
  • (Rod Outer Segment)

Abstract

Abstract Guanosine 3′,5′-cyclic monophosphate phosphodiesterase (EC 3.1.4.1) in frog rod outer segment prepared by a sucrose stepwise density gradient method was activated by light in the presence of GTP. Rhodopsin in rod outer segment was solubilized with sucrose laurylmonoester and then purified by concavanalin A-Sepharose column. Addition of photo-bleached preparation of the purified rhodopsin to the rod outer segment, which had been prepared by 43% (w/w) sucrose floatation, caused the activation of phosphodiesterase in the dark, while each component of the photo-product eluted from the column (all- trans retinal and opsin) did not. Regenerated rhodopsin prepared from 11- cis retinal and purified opsin activated phosphosdiesterase when it was bleached. From these facts it is suggested that an intermediate or a process of photolysis of rhodopsin causes activation of phosphodiesterase.

There are no comments yet on this publication. Be the first to share your thoughts.

Statistics

Seen <100 times
0 Comments

More articles like this

Activation of phosphodiesterase in frog rod outer...

on Biochimica et Biophysica Acta July 1981

Activation of phosphodiesterase in frog rod outer...

on Biochimica et Biophysica Acta... Jan 01, 1981

Activation of phosphodiesterase in frog rod outer...

on Biochimica et Biophysica Acta July 1981

Activation of phosphodiesterase in frog rod outer...

on Biochimica et Biophysica Acta... Jan 01, 1982
More articles like this..