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Activation of phosphodiesterase in frog rod outer segment by an intermediate of rhodopsin photolysis. I.

Authors
Journal
Biochimica et Biophysica Acta (BBA) - General Subjects
0304-4165
Publisher
Elsevier
Publication Date
Volume
675
Issue
2
Identifiers
DOI: 10.1016/0304-4165(81)90225-7
Keywords
  • Phosphodiesterase Activation
  • Rhodopsin Photolysis
  • Cyclic Gmp
  • (Rod Outer Segment)

Abstract

Abstract Guanosine 3′,5′-cyclic monophosphate phosphodiesterase (EC 3.1.4.1) in frog rod outer segment prepared by a sucrose stepwise density gradient method was activated by light in the presence of GTP. Rhodopsin in rod outer segment was solubilized with sucrose laurylmonoester and then purified by concavanalin A-Sepharose column. Addition of photo-bleached preparation of the purified rhodopsin to the rod outer segment, which had been prepared by 43% (w/w) sucrose floatation, caused the activation of phosphodiesterase in the dark, while each component of the photo-product eluted from the column (all- trans retinal and opsin) did not. Regenerated rhodopsin prepared from 11- cis retinal and purified opsin activated phosphosdiesterase when it was bleached. From these facts it is suggested that an intermediate or a process of photolysis of rhodopsin causes activation of phosphodiesterase.

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