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Fluid film method of electrophoresis

Authors
Journal
Journal of Laboratory and Clinical Medicine
0022-2143
Publisher
Elsevier
Publication Date
Keywords
  • Laboratory Methods
Disciplines
  • Biology
  • Design

Abstract

Abstract A procedure has been described for a method of electrophoresis designed for routine purposes and employing as an electrophoretic medium a thin fluid film. By layering the latter over a transparent vinyl sheet of suitable size, any convenient number of samples can be done simultaneously. By simply drying the film on the plastic after electrophoresis, the proteins are fixed for staining on a clear plastic background. The transparency of the plastic is an advantage for purposes of densitometric recording. By observing some simple precautions, consistent results can easily be obtained. Factors affecting the resolution of the method are discussed.

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