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Specificity of polyprenyl phosphates in thein vitroformation of lipid-linked sugars

Authors
Journal
Archives of Biochemistry and Biophysics
0003-9861
Publisher
Elsevier
Publication Date
Volume
181
Issue
2
Identifiers
DOI: 10.1016/0003-9861(77)90244-2
Keywords
  • Polysaccharides And Complex Carbohydrates
Disciplines
  • Chemistry

Abstract

Abstract Various polyprenyl phosphates were prepared by chemical phosphorylation of native and partially hydrogenated polyprenols. They were tested as lipid acceptors of sugars from nucleoside diphosphate sugars using a microsomal preparation from rat liver and membrane preparations from B. stearothermophilus, S. typhimurium, and Sh. flexneri. With the microsomal glycosyl transferase system, a demand for saturation of the α-isoprene residue of polyprenyl phosphate was observed; the chain length and cis/trans configuration of polyprenyl radical were less important. With bacterial glycosyl transferases, a demand for the unsaturated α-isoprene residue was observed. In B. stearothermophilus, the rate of synthesis of polyprenyl monophosphate glucose did not depend on the chain length of fully unsaturated polyprenyl phosphate. In S. typhimurium, C 55-polyprenyl phosphate was the most effective precursor of polyprenyl diphosphate galactose.

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