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Changes in circular dichroism and absorption spectrum of phycoerythrin chromophores by organic mercurial

Authors
Journal
Phytochemistry
0031-9422
Publisher
Elsevier
Publication Date
Volume
10
Issue
10
Identifiers
DOI: 10.1016/s0031-9422(00)89899-4
Disciplines
  • Biology

Abstract

Abstract When B- and R-phycoerythrins and their subunits are treated with high concentrations of an organic mercurial, all chromophores including the 500-nm chromophore are converted to a similar form exhibiting two absorption bands as well as two positive CD bands at 525 and 558 nm. This ultimate chromophore state is similar to that obtained from C-phycoerythrin treated in the same way. This adds further support to the contention that the different chromophore types are due to phycoerythrobilin residues located in three different protein environments. Upon removal of mercurial with mercaptoethanol, regeneration of the original types of chromophore is only partially effected.

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