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Carbon-14 biolabeling of flavanols and chlorogenic acids inCrataegus monogynacell suspension cultures

Authors
Journal
Food Chemistry
0308-8146
Publisher
Elsevier
Publication Date
Volume
105
Issue
2
Identifiers
DOI: 10.1016/j.foodchem.2006.11.065
Keywords
  • Crataegus Monogyna
  • Cell Suspension Cultures
  • Radiolabeling
  • Catechins
  • Proanthocyanidins
  • Chlorogenic Acids

Abstract

Abstract Pure radiolabeled phenolic compounds (including catechins, procyanidins and chlorogenic acids) were obtained by administrating 14C- l-phenylalanine to Crataegus monogyna cell suspension cultures. After a simple process of purification, pure compounds with high specific activities were recovered. In the present study, we describe a suitable method for obtaining labeled phenolics, which will be useful tools for in vivo feeding trials to trace their metabolic fate when consumed by animals and for in vitro activity mechanism studies.

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