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Amyloglucosidase immobilized on the surface of polyterephthalamide microcapsules containing multienzyme system with cofactor regeneration for the conversion of urea or ammonia to L-amino acids

McGill University
Publication Date
  • Chemistry
  • Pharmaceutical.
  • Engineering
  • Chemical.
  • Biology


Multienzyme systems with cofactor recycling have been immobilized within artificial cells (AC) to carry out certain metabolic functions in living cells. In liver failure there is an increase in systemic ammonia levels and a decrease in the ratio of branched chain (BCAA) to aromatic amino acids (AAA). The multienzyme system consisting of: urease, leucine dehydrogenase (LDH), glucose dehydrogenase (GDH) and immobilized coenzyme (dextran-NAD$ sp+$) is encapsulated in polyterephthalamide microcapsules with amyloglucosidase (AG) adsorbed on the surface: AG-MIC (1% albumin (BSA), Urease, LDH, GDH, dextran-NAD$ sp+$). Such AC may be administered orally where ammonia and urea would be removed while L-leucine, L-valine, L-isoleucine (BCAA) produced from the incorporation of ammonia to the corresponding $ alpha$-keto-acids would diffuse freely across the intestinal tract. Results show that 1 ml of 10% albumin filled AC with a mean diameter of 86.9 $ mu$m can adsorb a maximum of 94.87 mg of AG at PH = 8.0 and T = 37$ sp circ$C.

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