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Calcium ionophore A23187 induction of spermidine/spermineN1-acetyltransferase activity in bovine lymphocytes

Authors
Journal
FEBS Letters
0014-5793
Publisher
Wiley Blackwell (John Wiley & Sons)
Publication Date
Volume
188
Issue
2
Identifiers
DOI: 10.1016/0014-5793(85)80405-1
Keywords
  • Calcium Ionophore
  • Spermidine/Spermini N1-Acetyltransferase
  • Ornithine Decarboxylase
  • Enzyme Induction
  • Indomethacin
  • Bovine Lymphocyte
Disciplines
  • Biology

Abstract

Abstract The divalent cation ionophore A23187 increased the activity in bovine lymphocytes of spemidine/spermine N 1-acetyltransferase, a rate-limiting enzyme in polyamine biodegradation. The enzyme was induced in a dose- and time-dependent manner. Induction was suppressed by indomethacin, but not by trifluoperazine (TFP), N-(6-aminohexyl)-5-chloro-1-naphthalenesulfonamide (W-7) or palmitoylcarnitine. These results suggest that the activation of phospholipase A 2 involves the induction of spermidine/spermine N 1-acetyltransferase. Omithine decarboxylase, a rate-limiting enzyme in polyamine biosynthesis, was not suppressed by indomethacin but was by TFP and W-7. The molecular mechanism of the induction of spermidine/spermine N 1-acetyltransferase and ornithine decarboxylase may be different.

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