Abstract Theoretical exploration of education informatization construction is continuously specified in China. Education information ecosystem and education information system have been widely applied as a mature theory to guide education informationization construction. This thesis furthers the research into the structure of “Education Information Ecosystem”, and indicates that the structure is composed of three essential elements: education information subject, education information environment and education information resources, which interact with each other and form three kinds of relationships: the relationship between subjects and subjects, that between subjects and environment and that between environmental factors. The interaction between these elements and those three relationships constructs a three-dimensional Education Information Ecosystem structure of information chain, resources nets and knowledge domain with the oriention of the construction of wisdom circle. The thesis is thereby showing the direction for the informatization construction and development.