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Médicaments et aliments : approche ethnopharmacologique = Medicines and foods : ethnopharmacological approach

Authors
Publisher
ORSTOM
Publication Date
Keywords
  • Algue Marine
  • Extraction
  • Activite Biologique
  • Animal De Laboratoire
  • Experimentation Animale
  • Locomotion
  • Hypnotique
Disciplines
  • Biology
  • Medicine
  • Pharmacology

Abstract

Neuropharmacological activity of Cystoseira usneoïdes MÉDICAMENTS ET ALIMENTS : L'APPROCHE ETHNOPHARMACOLOGIQUE I 295 Neuropharrnacological activity of Cystoseira usneoïdes VAZQUEZ-FREIRE M.J., LAMELA M., ANCA J. and CALLEJA J.M. Department of Pharmacology, Pharmacy and Pharmaceutical Technology, Faculty of Pharmacy, University of Santiago de Compostela, Spain. INTRODUCTION In recent years, many studies have been canied out on marine organisms in order to discover possible pharmacological ac- tivities'. In this way, several marine seaweeds have been in- vestigated in OUT laboratory for their effects on glycemia and diabetes2S3. When an extractive solution from Cystoseira usneoides was assayed, no effect on blood glucose levels was noted, but a notorius quiescent behaviour a few minutes after administration was observed. A preliminary study was then canied out in order to investigate the possible neuropharma- cological effects of C. usneoides. MATERIALS AND METHODS Ph!EPARATION OF THE SEAWEED EXTRACT Cystoseira usneoides (L.) Roberts (Cystoseirncere) was hand- picked on Porto Nadelas beach (La Coruiia, Spain) at low tide. The fresh material was washed, cut into pieces and dried at 50 OC. The powderedmaterial was extracted with boiling metha- no1 (4 vol.) three times. The residual seaweed was then dried and extracted with 2% aqueous calcium chloride for 4 hours with stirring, twice at room temperature and once at 70 OC4. Filtered solutions were combined and concentrated in vacuo to a short volume. This solution was then poured into excess of ethanol and the resulting precipitate dissolved in water and dia- lyzed against distilled water. The non-dialyzed portion was fi- nally freeze-dried (percentage yield respect to initial dried algae = 4) and assayed at doses of 6.25, 12.5 and 25 mgkg. Animals Al1 experiments were carried out using Male CD-1 mice (25 k3 g), exc

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