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The Cyclization of Farnesyl Diphosphate and Nerolidyl Diphosphate by a Purified Recombinant δ-Cadinene Synthase1

Authors
Publisher
American Society of Plant Physiologists
Publication Date
Source
PMC
Keywords
  • Research Article
Disciplines
  • Biology

Abstract

The Cyclization of Farnesyl Diphosphate and Nerolidyl Diphosphate by a Purified Recombinant δ-Cadinene Synthase by Chauncey R. Benedict, Jia-Ling Lu, Donald W. Pettigrew, Jinggao Liu, Robert D. Stipanovic, and Howard J. Williams Plantphysiol Volume 125(4):1754-1765 April 1, 2001 ©2001 by American Society of Plant Biologists GC-MS analysis of the sesquiterpene products formed from the cyclization of (3RS)-[4,4,13,13,13-2H5]-NDP by recombinant CDN1-C1. Benedict C et al. Plantphysiol 2001;125:1754-1765 ©2001 by American Society of Plant Biologists Mass spectra of the sesquiterpenes formed from the cyclization of (3RS)-[4,4,13,13,13-2H5]-NDP by CDN1-C1. Benedict C et al. Plantphysiol 2001;125:1754-1765 ©2001 by American Society of Plant Biologists Proposed reaction mechanism for the cyclization of (1RS)-[1-2H](E,E)-FDP by CDN1-C1. Benedict C et al. Plantphysiol 2001;125:1754-1765 ©2001 by American Society of Plant Biologists Proposed reaction mechanism for the cyclization of (3RS)-[4,4,13,13,13-2H5]-NDP. Benedict C et al. Plantphysiol 2001;125:1754-1765 ©2001 by American Society of Plant Biologists Active site region of CDN1-C1 modeled on the TEAS structure. Benedict C et al. Plantphysiol 2001;125:1754-1765 ©2001 by American Society of Plant Biologists SDS-PAGE of purified recombinant CDN1-C1. Benedict C et al. Plantphysiol 2001;125:1754-1765 ©2001 by American Society of Plant Biologists GC-MS analysis of the sesquiterpene products formed from the cyclization of (3RS)-[4,4,13,13,13-2H5]-NDP by recombinant CDN1-C1. Mass spectra of the sesquiterpenes formed from the cyclization of (3RS)-[4,4,13,13,13-2H5]-NDP by CDN1-C1. A, [4,4,13,13-2H4]-(E)-β-farnesene. B, [6,6,15,15,15-2H5]-β-bisabolene. C, [8,8,15,15,15-2H5]-CDN. D, [6,6,15,15,15-2H5]-α-bisabolol. Proposed reaction mechanism for the cyclization of (1RS)-[1-2H](E,E)-FDP by CDN1-C1. Proposed reaction mechanism for the cyclization of (3RS)-[4,4,13,13,13-2H5]-NDP. Active site region of CDN1-C1 modeled on the TEAS structure. Amino a

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