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Unsterile and continuous production of polyhydroxybutyrate by Halomonas TD01

Authors
Journal
Bioresource Technology
0960-8524
Publisher
Elsevier
Volume
102
Issue
17
Identifiers
DOI: 10.1016/j.biortech.2011.05.068
Keywords
  • Halomonas
  • Halophilic Bacterium
  • Phb
  • Polyhydroxyalkanoates
  • Continuous Fermentation

Abstract

Abstract An unsterile and continuous fermentation process was developed based on a halophilic bacterium termed Halomonas TD01 isolated from a salt lake in Xinjiang, China. The strain reached 80g/L cell dry weight containing 80% poly(3-hydroxybutyrate) (PHB) on glucose salt medium during a 56h fed-batch process. In a 14-day open unsterile and continuous process, the cells grew to an average of 40g/L cell dry weight containing 60% PHB in the first fermentor with glucose salt medium. Continuous pumping of cultures from the first fermentor to the second fermentor containing the nitrogen-deficient glucose salt medium diluted the cells but allowed them to maintain a PHB level of between 65% and 70% of cell dry weight. Glucose to PHB conversions were between 20% and 30% in the first fermentor and above 50% in the second one. This unsterile and continuous fermentation process opens a new area for reducing the cost in polyhydroxyalkanoates production.

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