Affordable Access

Publisher Website

A novel radioimmunoassay for human erythropoietin using a synthetic NH2-terminal polypeptide and anti-peptide antibodies

Authors
Journal
Journal of Immunological Methods
0022-1759
Publisher
Elsevier
Publication Date
Volume
69
Issue
2
Identifiers
DOI: 10.1016/0022-1759(84)90316-8
Keywords
  • Peptide
  • Nh2-Terminus
  • Radioimmunoassay
  • Erythropoietin
  • Staphylococcus Aureus
  • Immunoprecipitation
  • Antibodies
Disciplines
  • Biology
  • Design

Abstract

Abstract Antibodies prepared by immunizing rabbits with a synthetic polypeptide representing the first 26 amino acids of the proposed NH 2-terminal sequence of human erythropoietin cross-reacr with peptide, highly purified 125I-labeled erythroppoietin and biologically active erythropoietin. We have now developed a rapid and convenient radioimmunoassay for the hormone using 125I-labeled peptide and anti-peptide antibodies. This peptide/anti-peptide approach completely circumvents the relative lack of availability of highly purified 125I-labeled erythropoietin and of erythropoietin for immunization purposes and makes a radioimmunoassay for the hormone universally available to investigators. This concept may be applied as a general method for designing radioimmunoassays of molecules available in severely restricted quantities.

There are no comments yet on this publication. Be the first to share your thoughts.