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A membrane-lytic immunoconjugate selective for human tumor T-lymphocytes

Authors
Journal
International Journal of Immunopharmacology
0192-0561
Publisher
Elsevier
Publication Date
Volume
20
Issue
9
Identifiers
DOI: 10.1016/s0192-0561(98)00053-8
Keywords
  • Cancer
  • Cytotoxin
  • Immunoconjugate
  • Membrane-Lytic Toxin
  • Monoclonal Antibody

Abstract

Abstract An immunoconjugate was constructed from a monoclonal antibody recognizing human T-lymphoblastoid cells and a membrane-lytic cytotoxin purified from the venom of the Thailand cobra. Activities of this novel immunoconjugate were compared using human and murine T-lymphocyte cell lines. The ability of the conjugate to inhibit human T-cell proliferation, as measured by incorporation of [ 3H]thymidine, was three to four times higher than its ability to inhibit proliferation of mouse L1210 cells. The immunoconjugate EC 50 for human CEM cells was equivalent to 0.1 nmoles per 2×10 5 target cells. Immunoconjugate selectivity paralleled the monoclonal antibodys binding characteristics. Preincubation with free antibody blocked the effect of the conjugate, but only upon the human target cells. This study supports the feasibility of directing a toxic moiety to the surface of a cancer cell to accomplish cell destruction without requiring prior toxin internalization and uncoupling from its antibody carrier.

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