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The measurement of protein synthesis in biological systems

Authors
Journal
Life Sciences
0024-3205
Publisher
Elsevier
Publication Date
Volume
30
Issue
20
Identifiers
DOI: 10.1016/0024-3205(82)90300-9
Disciplines
  • Biology

Abstract

Abstract Attempts to quantitate metabolism in the lung and other tissues using radioactive precursors may be subject to significant errors arising from inappropriate assumptions regarding precursor metabolism, compartmentation and specific radioactivity. This article reviews the type and magnitude of error which may complicate such measurements by presenting specific data from experiments using radioactive amino acids to estimate the rate of protein synthesis. The applicability of these observations to other metabolic systems is discussed briefly in order to develop a more general awareness of the errors which may result from incomplete validation of experimental measurements using radioisotopes.

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