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C4 allotyping on plasma or serum: application to routine laboratories.

Authors
Type
Published Article
Journal
Human Immunology
0198-8859
Publisher
Elsevier
Publication Date
Volume
21
Issue
3
Pages
165–171
Identifiers
PMID: 3131277
Source
Medline
License
Unknown

Abstract

Allotypes of the fourth component of complement (C4) can be detected by electrophoresis and immunofixation after treatment of EDTA plasma with neuraminidase (NAse). We have assessed the value of additional treatment with carboxypeptidase B (CPseB). Following treatment with CPseB + NAse, each allele is resolved into a single band, permitting clear definition of overlapping bands seen following treatment with NAse alone. More importantly, C4 allotypes can be determined using stored heparinized plasma or serum. Most C4 null alleles can be assigned without requiring family studies. The approach described is suitable for routine use by tissue typing laboratories.

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