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The Distribution of Mitoses in a Fibroblast Colony

Authors
Journal
British Journal of Cancer
0007-0920
Publisher
Nature Publishing Group
Publication Date
Keywords
  • Articles
Disciplines
  • Chemistry
  • Medicine

Abstract

'300 THE DISTRIBUTION OF MITOSES IN'A FIBROBLAST COLONY. A. F. PHILLIPS.* From the Department of Radiotherapeutics, University of Cambridge. Received for publication June 3, 1952. FOR the development of quantitative methods of study of mitotic inhibitionby X-rays and chemical a ents, it has been necessary to examine the distribution of mitoseg in tissue cultures'of chick fibroblasts. AU the resting and mitotic cells forming the outgrowth of a typical fibroblast colony have been counted and their topographical distribution recorded. A statistical analysis of the distri-bution of,the mitoses has been made. Previous quantitative work on tissue culture colonies in vitro has been re- viewed by Mayer (1939). Afitoses in fibroblast colonies were mapped by Fischer and Parker (1929), by'GaiRard (1935), by Jacoby (1937), and in epithelial coloniesby Ephrussi and Litvae (1934). Of these only GaiRard's and Jacoby's maps aredirectly comparable with the present work. WiRmer (1933) studied the relation- ship between ceR den's'ity and frequency of mitosis. Since 1939 the results of extensive studies of fibroblast colonies grown in a two-phase medium have been published by Cunningham and Kirk (1942) and Tompkins, Cunningham and Kirk(1947), but these colonies are not directly comparable with " hanging drop colonies. MATERIAL AND METHODS. The culture studied was kindly supphed by Mrs. 1. Simon-Reuss. It was a hanging drop colony of the 4th passage from a culture of fibroblasts from the choroid and sclerotic of an 11-day chick embryo. The colony had been grow-nfor 24 hours in the usual 'mixture of one drop of fowl plasma and one drop of 15 per cent chick embryo extract' then fixed in Susa, stained with Heidenhain's haematoxyhn, and mounted whole. It was selected from a number of " control colonies of another experiment, asbeing of average growth and mitot-ic activity, and free from any obvious abnormahty. The colony was mapped using the 1/6 inch (dry) objective and an eye-piece micrometer which divided th

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