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Cross-inhibitory activity of cereal protein inhibitors against alpha-amylases and xylanases

Authors
Publisher
Elsevier BV
Publication Date
Keywords
  • The Purification And Characterisation Of A Xylanase Inhibitor (Xip-I) From Wheat Was Reported Previo
  • In Our Current Work
  • Xip-I Is Also Demonstrated To Have The Capacity To Inhibit The Two Barley Alpha-Amylase Isozymes (Am
  • Xip-I Completely Inhibited The Activity Of Amy1 And Amy2 Towards Insoluble Blue Starch And A Soluble
  • A Ternary Complex Was Formed Between Insoluble Starch
  • A Catalytically Inactive Mutant Of Amy1 (D180A)
  • And Xip-I
  • Suggesting That The Substrate-Xip-I Interaction Is Necessary For Inhibition Of Barley Alpha-Amylases
  • K(I) Values For Alpha-Amylase Inhibition
  • However
  • Could Not Be Calculated Due To The Nonlinear Nature Of The Inhibition Pattern
  • Furthermore
  • Surface Plasmon Resonance And Gel Electrophoresis Did Not Indicate Interaction Between Xip-I And The
  • The Inhibition Was Abolished By Cacl(2)
  • Indicating That The Driving Force For The Interaction Is Different From That Of Complexation Between
  • This Is The First Report Of A Proteinaceous Inhibitor Of Amy1
  • Basi
  • In Addition
  • Was Demonstrated To Partially Inhibit The Endo-1
  • 4-Beta-D-Xylanase From Aspergillus Niger (Xyla) Of Glycoside Hydrolase Family 11
  • Taken Together
  • The Data Demonstrate For The First Time The Dual Target Enzyme Specificity Of Basi And Xip-I Inhibit
Disciplines
  • Biology

Abstract

Cross-inhibitory activity of cereal protein inhibitors against alpha-amylases and xylanases - DTU Orbit (15/04/14) Cross-inhibitory activity of cereal protein inhibitors against alpha-amylases and xylanases - DTU Orbit (15/04/14) Sancho, A. I.; Faulds, C. B.; Juge, N.; Svensson, Birte; Bartolomé, B.; Williamson, G. / Cross-inhibitory activity of cereal protein inhibitors against alpha-amylases and xylanases. In: Biochimica et Biophysica Acta, Vol. 1650, No. 1-2, 2003, p. 136-144. Publication: Research - peer-review › Journal article – Annual report year: 2003

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