Abstract DNA typing was performed on 155 unrelated volunteers from Parana (South Brazil) using 9 non-CODIS mini-short tandem repeat (miniSTR) markers (D10S1248, D14S1434, D22S1045, D1S1677, D2S441, D4S2364, D20S482, D3S3053 and D6S474), comprising three different multiplex systems. The allele frequencies, heterozygosity, power of discrimination, mean exclusion chance, polymorphism information content and typical paternity index of all loci were calculated by statistical analysis. The combined matching probability and the combined mean exclusion chance was 1 in 9,09 × 107 and 0.995856, respectively. The typical paternity index varied from 1077 (D4S2364) to 2588 (D3S3053). No evidence of deviation from Hardy–Weinberg equilibrium was observed for any loci, except for D10S1248 ( p value = 0.00101). There was also no evidence for correlation of alleles between loci. Five of the six loci showed good levels of polymorphisms, with heterozygosities greater than 0.65. This study demonstrates that these multiplex systems are useful and convenient tools for forensic identification and parentage testing in the Brazilian population of Parana.