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Development of a two-site enzyme-linked immunosorbent assay for quantification of the flagellar antigen in blackleg vaccines

Authors
Journal
Journal of Microbiological Methods
0167-7012
Publisher
Elsevier
Publication Date
Volume
31
Identifiers
DOI: 10.1016/s0167-7012(97)00089-4
Keywords
  • Blackleg Vaccine
  • Clostridium Chauvoei
  • Elisa
  • Flagellar Antigen
Disciplines
  • Biology

Abstract

Abstract A quantitative two-site enzyme-linked immunosorbent assay (two-site ELISA) procedure using a protective monoclonal antibody against the flagella of Clostridium chauvoei has been developed to measure a flagellar concentration in blackleg vaccines. The two-site ELISA was highly specific for flagella of C. chauvoei, and had a detection limit of approximately 0.125 μg ml −1 of flagella. With this assay, the flagella level in blackleg vaccines could be measured and will be available for quality controls of vaccines.

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