Affordable Access

Publisher Website

7. Adsorption chromatography

DOI: 10.1016/s0301-4770(08)61411-6


Publisher Summary This chapter describes the different aspects of adsorption chromatography. Adsorption chromatography utilizes the ability of solid stationary phases to adsorb individual components from mixtures to different extents. The adsorption constant describes the relationship detected in recording adsorption isotherms: the amount of substance bonded per gram of adsorbent is referred to the concentration of the solution. The effective area covered by the substance molecule can be calculated from the monolayer covering the adsorbent surface, and results from the quotient of the specific surface area of the adsorbent and the amount of substance adsorbed per gram. The flow of an eluent mixture over an adsorbent may itself be considered a chromatographic process in which one of the components is the sample while the other is the eluent. The higher the probability for a substance to be in the stationary phase, the longer it is retained. Polar components are retained by polar adsorbents in which the retention increases with decreasing polarity of the eluent. Combinations of an organic liquid and water are frequently used in reversed-phase chromatography. The chapter also describes the role of solubility parameters in chromatographic processes.

There are no comments yet on this publication. Be the first to share your thoughts.


Seen <100 times

More articles like this

Isolation of Individual Fullerenes C60and C70by Ex...

on Mendeleev Communications Jan 01, 1993

Adsorption chromatography.

on Methods in medical research 1970

Separation of Delta5- and Delta7-phytosterols by a...

on Journal of Agricultural and Fo... Feb 22, 2006

An isocratic high-performance liquid chromatograph...

on Methods in Molecular Biology 2006
More articles like this..