Publisher Summary This chapter describes the different aspects of adsorption chromatography. Adsorption chromatography utilizes the ability of solid stationary phases to adsorb individual components from mixtures to different extents. The adsorption constant describes the relationship detected in recording adsorption isotherms: the amount of substance bonded per gram of adsorbent is referred to the concentration of the solution. The effective area covered by the substance molecule can be calculated from the monolayer covering the adsorbent surface, and results from the quotient of the specific surface area of the adsorbent and the amount of substance adsorbed per gram. The flow of an eluent mixture over an adsorbent may itself be considered a chromatographic process in which one of the components is the sample while the other is the eluent. The higher the probability for a substance to be in the stationary phase, the longer it is retained. Polar components are retained by polar adsorbents in which the retention increases with decreasing polarity of the eluent. Combinations of an organic liquid and water are frequently used in reversed-phase chromatography. The chapter also describes the role of solubility parameters in chromatographic processes.