Affordable Access

Publisher Website

Allosteric inhibition ofDictyostelium discoideumfructose-1,6-bisphosphatase by fructose 2,6-bisphosphate

Authors
Journal
FEBS Letters
0014-5793
Publisher
Wiley Blackwell (John Wiley & Sons)
Publication Date
Volume
241
Identifiers
DOI: 10.1016/0014-5793(88)81029-9
Keywords
  • Fructose-1
  • 6-Bisphosphatase
  • Fructose 2
  • 6-Bisphosphate
  • (Dictyostelium Discoideum)
Disciplines
  • Biology

Abstract

Abstract It has been found that the inhibition of Dictyostelium discoideum fructose-1,6-bisphosphatase by fructose 2,6-P 2 greatly diminished when the pH was raised to the range 8.5–9.5, which resulted in a marked decrease of the affinity for the inhibitor with no change in the K m for the substrate. This provides evidence for the involvement of an allosteric site for fructose 2,6-P 2. Moreover, the fact that excess substrate inhibition also decreased at the pH values for minimal fructose 2,6-P 2 inhibition, and was essentially abolished in the presence of fructose 2,6-P 2, strongly suggests that this inhibition takes place by binding of fructose 1,6-P 2 as a weak analogue of the physiological effector fructose 2,6-P 2.

There are no comments yet on this publication. Be the first to share your thoughts.