Publisher Summary The plasma lipids are associated with specific proteins to form lipoprotein complexes of various sizes. This chapter provides an overview on the important role of laboratory techniques that they have played in the study of lipoproteins and apolipoproteins, and discusses the invaluable aid provided by a number of fascinating human genetic variants, such as abeta- and hypobetalipoproteinemia, Tangier disease, and apoC-II deficiency. The studies of these disorders have generated important structural and functional insights and also stimulated technological advances. In its analytical and various preparative modes, ultracentrifugation has helped in many important developments in the plasma lipoprotein field both from the structural and metabolic standpoints and still retains a dominant role aided by the developments in ultracentrifuge models and rotors. Another physical method, which has aided in the studies of plasma lipoproteins, has been electrophoresis. However, besides the physical methods, column chromatography in beds of various pore sizes either alone or in combination with ultracentrifugal and electrophoretic techniques has also acquired an important place in the study of plasma lipoproteins.