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Crystallization and preliminary crystallographic analysis of an <em>Escherichia coli</em>-selected mutant of the nuclease domain of the metallonuclease colicin E7

Authors
Publisher
Wiley-Blackwell Munksgaard
Publication Date
Keywords
  • <P>The Metallonuclease Colicin E7 Is A Member Of The Hnh Family Of Endonucleases
  • It Serves As A Bacterial Toxin In <Em>Escherichia Coli</Em>
  • Protecting The Host Cell From Other Related Bacteria And Bacteriophages By Degradation Of Their Chro
  • Its Cell-Killing Activity Is Attributed To The Nonspecific Nuclease Domain (Ncole7)
  • Which Possesses The Catalytic <Em>ββα</Em>-Type Metal Ion-Binding Hnh Motif At Its C-Terminus
  • Mutations Affecting The Positively Charged Amino Acids At The N-Terminus Of Ncole7 (444-576) Surpris
  • (2013)
  • J
  • Biol
  • Inorg
  • Chem
  • 18
  • 309-321]
  • The Necessity Of The N-Terminal Amino Acids For The Function Of The C-Terminal Catalytic Centre Pose
  • Precise Knowledge Of The Intramolecular Interactions Of These Residues That Affect The Catalytic Act
  • In This Study
  • The N-Terminal Deletion Mutant ΔN4-Ncole7-C* Of The Nuclease Domain Of Colicin E7 Selected By E
  • <Em>Coli</Em> Was Overexpressed And Crystallized At Room Temperature By The Sitting-Drop Vapour-Diff
  • X-Ray Diffraction Data Were Collected To 1
  • 6 Å Resolution And Could Be Indexed And Averaged In The Trigonal Space Group P3<Sub>1</Sub>21 Or P3
  • With Unit-Cell Parameters <Em>A</Em> = <Em>B</Em> = 55
  • 4
  • <Em>C</Em> = 73
  • 1 Å
  • Structure Determination By Molecular Replacement Is In Progress
  • </P>

Abstract

Crystallization and preliminary crystallographic analysis of an Escherichia coli-selected mutant of the nuclease domain of the metallonuclease colicin E7 - DTU Orbit (25/04/14) Crystallization and preliminary crystallographic analysis of an Escherichia coli-selected mutant of the nuclease domain of the metallonuclease colicin E7 - DTU Orbit (25/04/14) Czene, Aniko et al."Crystallization and preliminary crystallographic analysis of an Escherichia coli-selected mutant of the nuclease domain of the metallonuclease colicin E7". Acta Crystallographica. Section F: Structural Biology and Crystallization Communications Online. 2013, F69(5). 551-554. Available: 10.1107/S1744309113008233

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