Affordable Access

Publisher Website

Integrase and integration: biochemical activities of HIV-1 integrase

Authors
Journal
Retrovirology
1742-4690
Publisher
Springer (Biomed Central Ltd.)
Publication Date
Volume
5
Issue
1
Identifiers
DOI: 10.1186/1742-4690-5-114
Keywords
  • Review
Disciplines
  • Biology
  • Medicine

Abstract

Integration of retroviral DNA is an obligatory step of retrovirus replication because proviral DNA is the template for productive infection. Integrase, a retroviral enzyme, catalyses integration. The process of integration can be divided into two sequential reactions. The first one, named 3'-processing, corresponds to a specific endonucleolytic reaction which prepares the viral DNA extremities to be competent for the subsequent covalent insertion, named strand transfer, into the host cell genome by a trans-esterification reaction. Recently, a novel specific activity of the full length integrase was reported, in vitro, by our group for two retroviral integrases (HIV-1 and PFV-1). This activity of internal cleavage occurs at a specific palindromic sequence mimicking the LTR-LTR junction described into the 2-LTR circles which are peculiar viral DNA forms found during viral infection. Moreover, recent studies demonstrated the existence of a weak palindromic consensus found at the integration sites. Taken together, these data underline the propensity of retroviral integrases for binding symmetrical sequences and give perspectives for targeting specific sequences used for gene therapy.

There are no comments yet on this publication. Be the first to share your thoughts.

Statistics

Seen <100 times
0 Comments

More articles like this

In vitro biochemical activities associated with re...

on Biochemical Society Transactio... February 1993

Retroviral integrase proteins and HIV-1 DNA integr...

on Journal of Biological Chemistr... Nov 30, 2012

The 156KELK159 tetrapeptide of HIV-1 integrase is...

on Molecular Biology Reports January 2012
More articles like this..