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Subcellular distribution of testosterone in rat prostate and its possible relationship to nuclear ribonucleic acid synthesis

Authors
Journal
Archives of Biochemistry and Biophysics
0003-9861
Publisher
Elsevier
Publication Date
Volume
124
Identifiers
DOI: 10.1016/0003-9861(68)90300-7
Disciplines
  • Biology

Abstract

Abstract The template capacity of rat prostatic whole nuclei from testosterone-implanted castrates was higher than that of castrates when assayed using an Escherchia coli RNA polymerase system. Attempts to localize this increased template activity in the “euchromatin” or “heterochromatin” fractions were unsuccessful. The E. coli RNA polymerase enzyme appeared to be saturated by an extremely low concentration of chromatin DNA. Both “heterochromatin” and “euchromatin” fractions are heavily repressed, and can be activated by the removal of histones. Prostatic “heterochromatin,” “euchromatin,” and intact nuclei in vivo and intact nuclei in vitro isolated from castrated rats take up more testosterone- 3H than the corresponding fractions obtained from testosterone-implanted castrated rats. In vivo this increased uptake is observed 2 hours after castration, and in vitro it is inhibited by 2,4-dinitrophenol. Specific uptakes of testosterone- 3H by the “euchromatin” fraction in vivo appeared to be higher than those onto “heterochromatin.”

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