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Optimum extraction of polysaccharides from Opuntia dillenii and evaluation of its antioxidant activities

Authors
Journal
Carbohydrate Polymers
0144-8617
Publisher
Elsevier
Publication Date
Volume
97
Issue
2
Identifiers
DOI: 10.1016/j.carbpol.2013.05.057
Keywords
  • Opuntia Dillenii
  • Polysaccharides
  • Extraction
  • Antioxidant Activity
  • Response Surface Methodology
Disciplines
  • Design

Abstract

Abstract Response surface methodology (RSM) was applied to optimize the extraction of crude polysaccharides from Opuntia dillenii (Ker–Gaw) Haw. A three-level, four-variable Box–Behnken design was employed to obtain the best possible combination of extraction temperature (80–90°C), extraction time (50–70min), number of extraction cycle (1–3 times), and ratio of water to raw material (8:1–12:1, v/w) for maximum yield of crude polysaccharide. Besides, the antioxidant capacity of crude polysaccharide was evaluated by DPPH assay. The results showed that optimized extraction conditions were extraction temperature 85°C, extraction time 63.7min, extraction 2 times and ratio of water to raw material 11.14:1. Under these conditions, the experimental yield was 27.36±0.21%, which is well in close agreement with the value (27.44%) predicted by RSM model. Pharmacological test showed that O. dillenii crude polysaccharides had a good antioxidant activity.

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